Acanthaceae is firmly within Lamiales.
Herbs, shrubs or twiners with stems that are often angled or winged. Usually with distinct ridges around the stem at the nodes. Stems and leaves often with cystoliths looking like small white rods. Leaves opposite. No stipules. Bracts and bractioles in the inflorescence. Calyx 4-5 lobed and fused at least at the base. Calyx often zygomorphic with unequal lobes. Four stamens or two stamens. Fruit a 2-locular loculicidal capsule (splitting lengthways).
Mendoncia
Fruit a 1-seeded drupe.
Saintpauliopsis
Retinacula lacking or papilliform, bracts alternate and fused to pedicels, corolla distinctly two lipped and small (less than 1cm), plant with trailing stems
Anisosepalum
Retinacula lacking or papilliform, bracts alternate and fused to pedicels, corolla distinctly two lipped and longer than 1cm, plant with erect stems
Elytraria
Retinacula lacking or papilliform, bracts alternate and not fused to pedicels, corolla almost radially symmetrical, leaves in basal or apical rosette, calyx 5-lobed.
Nelsonia
Retinacula lacking or papilliform, bracts alternate and not fused to pedicels, corolla almost radially symmetrical, leaves dispersed along elongated stems, calyx 4-lobed.
Pseudocalyx
Retinacula lacking or papilliform, bracts opposite, calyx a rim with many lobes, capsule more than 1.5cm long with a long beak, stellate hairs, anthers opening with apical pores.
Thunbergia
Retinacula lacking or papilliform, bracts opposite, calyx a rim with many lobes, capsule more than 1.5cm long with a long beak, anthers opening by slits.
Crossandra
Retinacula prominent. Four fertile stamens, anthers with 1 thecae, plant without cystoliths, stamens included in corolla tube, corolla hairy on outside
Stenandrium
Retinacula prominent. Four fertile stamens, anthers with 1 thecae, plant without cystoliths, stamens included in corolla tube, corolla glabrous on outside, leaf base cordate
Sclerochiton
Retinacula prominent. Four fertile stamens, anthers with 1 thecae, plant without cystoliths, stamens exserted with flattened bony filaments, calyx with 5 lobes.
Acanthus
Retinacula prominent. Four fertile stamens, anthers with 1 thecae, plant without cystoliths, stamens exserted with flattened bony filaments, calyx with 4 lobes, all four filaments similar
Blepharis
Retinacula prominent. Four fertile stamens, anthers with 1 thecae, plant without cystoliths, stamens exserted with flattened bony filaments, calyx with 4 lobes, leaves in whorls of four, two filaments flattened, seeds with hygroscopic hairs
Lepidagathis
Retinacula prominent. Four fertile stamens, anthers with 2 thecae, plant with cystoliths, calyx 4-lobed, inflorescence distincly dorsiventral with flowers and bracts on one side and sterile bracts on the other side, lower lip of corolla 3-lobed.
Barleria
Retinacula prominent. Four or two fertile stamens, anthers with 2 thecae, plant with cystoliths, calyx 4-lobed, lower lip of corolla never 3-lobed.
Duosperma
Retinacula prominent. Four fertile stamens, anthers with 2 thecae, plant with cystoliths,calyx 5-lobed, capsule with two seeds
Asystasia
Retinacula prominent. Four fertile stamens, anthers with 2 thecae, plant with cystoliths, calyx 5-lobed, capsule with four seeds, flowers in 1-sided racemose cymes.
Crabbea
Retinacula prominent. Four fertile stamens, anthers with 2 thecae, plant with cystoliths, calyx 5-lobed, capsule with four seeds, flowers in globose heads with large bracts that are sometimes spiny
Ruellia
Retinacula prominent. Four fertile stamens, anthers with 2 thecae, plant with cystoliths, calyx 5-lobed, capsule with four or more seeds, corolla radially symetrical
Hygrophila
Retinacula prominent. Four or two fertile stamens, anthers with 2 thecae, plant with cystoliths, calyx 5-lobed, capsule with four or more seeds, corolla distinctly two lipped, flowers in dense heads surrounded by spiny modified branches
Dyschoriste
Retinacula prominent. Four or two fertile stamens, anthers with 2 thecae, plant with cystoliths, calyx 5-lobed, capsule with four seeds, corolla distinctly two lipped, lower lip with stiff retrorse hairs
Whitfieldia
Retinacula prominent. Four fertile stamens, anthers with 2 thecae, plant with cystoliths, calyx 5-lobed, capsule with four seeds, flowers single, each supported by a single bract, corolla over 3cm long, seeds glabrous
Ruspolia
Retinacula prominent. Two fertile stamens, glabrous seeds, stamens with 1-thecous anthers, stamens included in corolla tube or not much exserted
Dicliptera
Retinacula prominent. Two fertile stamens, glabrous seeds, stamens with 2-thecous anthers, whole inflorescence cymes held between two bracts
Isoglossa
Retinacula prominent. Two fertile stamens, glabrous seeds, stamens with 2-thecous anthers, each flower supported by a single bract, the two anther thecae attached at the same height or at different heights
Rhinacanthus
Retinacula prominent. Two fertile stamens, glabrous seeds, stamens with 2-thecous anthers, each flower supported by a single bract, the two anther thecae attached at different heights
Justicia
Retinacula prominent. Two fertile stamens, glabrous seeds, stamens with 2-thecous anthers, each flower supported by a single bract, the two anther thecae attached at different heights, stamens exserted, lower anther theca with distinct appendage